全文获取类型
收费全文 | 9097篇 |
免费 | 838篇 |
国内免费 | 15篇 |
出版年
2023年 | 38篇 |
2021年 | 136篇 |
2020年 | 115篇 |
2019年 | 144篇 |
2018年 | 197篇 |
2017年 | 187篇 |
2016年 | 266篇 |
2015年 | 479篇 |
2014年 | 485篇 |
2013年 | 598篇 |
2012年 | 730篇 |
2011年 | 660篇 |
2010年 | 432篇 |
2009年 | 413篇 |
2008年 | 572篇 |
2007年 | 515篇 |
2006年 | 455篇 |
2005年 | 418篇 |
2004年 | 390篇 |
2003年 | 352篇 |
2002年 | 324篇 |
2001年 | 184篇 |
2000年 | 168篇 |
1999年 | 132篇 |
1998年 | 81篇 |
1997年 | 66篇 |
1996年 | 50篇 |
1995年 | 76篇 |
1994年 | 44篇 |
1993年 | 52篇 |
1992年 | 86篇 |
1991年 | 75篇 |
1990年 | 80篇 |
1989年 | 83篇 |
1988年 | 80篇 |
1987年 | 76篇 |
1986年 | 64篇 |
1985年 | 73篇 |
1984年 | 52篇 |
1983年 | 44篇 |
1982年 | 34篇 |
1980年 | 29篇 |
1979年 | 47篇 |
1978年 | 43篇 |
1977年 | 29篇 |
1976年 | 41篇 |
1975年 | 32篇 |
1974年 | 32篇 |
1973年 | 26篇 |
1972年 | 24篇 |
排序方式: 共有9950条查询结果,搜索用时 31 毫秒
71.
Jihun Ryu Do Un Hwang Yeon Jae Bae Si Hyeock Lee Young Ho Kim Seung‐Yeol Lee Hee‐Young Jung Kwang Shik Choi 《Entomological Research》2019,49(9):432-435
Pyrethroid insecticides have been effective and powerful for controlling mosquitoes. However, abuse of these insecticides increases the number of resistant mosquitoes. In this study, Culex pipiens pallens and Aedes koreicus were collected from an artificial reservoir in the vicinity of a populated area in Korea, which is also a migratory bird catchment area. To monitor resistance to pyrethroid insecticides in mosquitoes, genomic DNA from the collected mosquitoes was sequenced for the kdr mutation in the voltage‐gated sodium channel (VGSC) gene. As a result, three samples with homozygous resistance (17.6%) and one with heterozygous resistance (5.9%) were found among 17 Cx. pipiens pallens specimens. One of the samples had a unique sequence at the amplified VGSC region. Of the 15 Ae. koreicus, no insecticide resistant individuals were found. In Korea, this is the first report of kdr genetic traits in Ae. koreicus and Cx. pipiens pallens and of a unique VGSC allele in Cx. pipiens pallens. Further investigation is needed to monitor the kdr resistance of these species in Korea and to determine how the unique sequence found in Cx. pipiens pallens is related to insecticide resistance. 相似文献
72.
Haoran Zhang Zachary A. Steelman Derek S. Ho Kengyeh K. Chu Adam Wax 《Journal of biophotonics》2019,12(2)
In recent years, significant work has been devoted to the use of angle‐resolved elastic scattering for the extraction of nuclear morphology in tissue. By treating the nucleus as a Mie scattering object, techniques such as angle‐resolved low‐coherence interferometry (a/LCI) have demonstrated substantial success in identifying nuclear alterations associated with dysplasia. Because optical biopsies are inherently noninvasive, only a small, discretized portion of the 4π scattering field can be collected from tissue, limiting the amount of information available for diagnostic purposes. In this work, we comprehensively characterize the diagnostic impact of variations in angular sampling, range and noise for inverse light scattering analysis of nuclear morphology, using a previously reported dataset from 40 patients undergoing a/LCI optical biopsy for cervical dysplasia. The results from this analysis are applied to a benchtop scanning a/LCI system which compromises angular range for wide‐area scanning capability. This work will inform the design of next‐generation optical biopsy probes by directing optical design towards parameters which offer the most diagnostic utility. 相似文献
73.
74.
75.
76.
Zheng Li Xingye Li Xin Chen Shugang Li Idy H. T. Ho Xiaodong Liu Matthew T. V. Chan William K. K. Wu 《Cell proliferation》2019,52(1)
Neuropathic pain, a type of chronic and potentially disabling pain resulting from primary injury/dysfunction of the somatosensory nervous system and spinal cord injury, is one of the most intense types of chronic pain, which incurs a significant economic and public health burden. However, our understanding of its cellular and molecular pathogenesis is still far from complete. Long non‐coding RNAs (lncRNAs) are important regulators of gene expression and have recently been characterized as key modulators of neuronal functions. Emerging evidence suggested that lncRNAs are deregulated and play pivotal roles in the development of neuropathic pain. This review summarizes the current knowledge about the roles of deregulated lncRNAs (eg, KCNA2‐AS, uc.48+, NONRATT021972, MRAK009713, XIST, CCAT1) in the development of neuropathic pain. These studies suggested that specific regulation of lncRNAs or their downstream targets might provide novel therapeutic avenues for this refractory disease. 相似文献
77.
Ho Shin Kim Van-Hai Hoang Mannkyu Hong Kyung Chul Kim Jihyae Ann Cong-Truong Nguyen Ji Hae Seo Hoon Choi Jun Yong Kim Kyu-Won Kim Woong Sub Byun Sangkook Lee Seungbeom Lee Young-Ger Suh Jie Chen Hyun-Ju Park Tae-Min Cho Ji Young Kim Jeewoo Lee 《Bioorganic & medicinal chemistry》2019,27(7):1370-1381
On the basis of deguelin, a series of the B,C-ring truncated surrogates with N-substituted amide linkers were investigated as HSP90 inhibitors. The structure activity relationship of the template was studied by incorporating various substitutions on the nitrogen of the amide linker and examining their HIF-1α inhibition. Among them, compound 57 showed potent HIF-1α inhibition and cytotoxicity in triple-negative breast cancer lines in a dose-dependent manner. Compound 57 downregulated expression and phosphorylation of major client proteins of HSP90 including AKT, ERK and STAT3, indicating that its antitumor activity was derived from the inhibition of HSP90 function. The molecular modeling of 57 demonstrated that 57 bound well to the C-terminal ATP-binding pocket in the open conformation of the hHSP90 homodimer with hydrogen bonding and pi-cation interactions. Overall, compound 57 is a potential antitumor agent for triple-negative breast cancer as a HSP90 C-terminal inhibitor. 相似文献
78.
Meeja Seo Jeong Hwan Kim Bo Yoon Seo Changgyu Park Byeong Ryeol Choi Kwang Ho Kim Chang Woo Ji Jum Rae Cho 《Journal of Asia》2019,22(4):1013-1018
Anastatus orientalis is a solitary endoparasitoid of Lycorma delicatula (Hemiptera: Fulgoridae) eggs. We investigated the development, longevity, fecundity, and sex ratio of A. orientalis on different temperatures to establish the optimal temperature condition for laboratory mass rearing. There were significant differences in its development and longevity between 15 °C and the rest of temperature conditions (20, 25, and 30 °C), among which were no significant differences. The average number of eggs laid by A. orientalis was higher at 20 and 25 °C, but there was no statistically significant difference in its fecundity between the two temperatures. More females emerged at 15 and 20 °C than higher temperatures.Parasitisms of A. orientalis varied with host egg ages and densities. Even in 14 day old eggs of L. delicatula, parasitoids successfully emerged from 92.3% of the parasitized eggs. On the other hand, parasitism was lowest (13.8%) on just before hatching eggs. Parasitism was negatively dependent on host density.Oviposition behavior for A. orientalis primarily occurred at 1400–1600 h, not at 2400–0800 h. The majority of this parasitoid's emergence occurred at 0800–1000 h, largely before noon. These results may be useful for controlling the adequate time for supplying host eggs and release density of this parasitoid as well as for potentially predicting the accurate time for securing parasitoid adults in laboratory mass rearing of A. orientalis. 相似文献
79.
80.
Saranna Fanning Aftabul Haque Thibaut Imberdis Valeriya Baru M. Inmaculada Barrasa Silke Nuber Daniel Termine Nagendran Ramalingam Gary P.H. Ho Tallie Noble Jackson Sandoe Yali Lou Dirk Landgraf Yelena Freyzon Gregory Newby Frank Soldner Elizabeth Terry-Kantor Tae-Eun Kim Dennis Selkoe 《Molecular cell》2019,73(5):1001-1014.e8